The sequence of reagents used in Gram staining is ()
A: Safranin solution → iodine solution → alcohol →
crystal violet
B: crystal violet → alcohol → iodine solution → Safranin
solution
C: Crystal violet → iodine solution → alcohol → Safranin
solution
D: Safranin solution → alcohol → crystal violet → iodine
solution
E: Safranin solution → crystal violet → iodine solution →
alcohol
A: Safranin solution → iodine solution → alcohol →
crystal violet
B: crystal violet → alcohol → iodine solution → Safranin
solution
C: Crystal violet → iodine solution → alcohol → Safranin
solution
D: Safranin solution → alcohol → crystal violet → iodine
solution
E: Safranin solution → crystal violet → iodine solution →
alcohol
举一反三
- Which of the following staining solution are used in the bacterial Gram staining method？ ( ) A: Crystal violet B: Iodine solution C: Ethanol D: Safranin
- The reagents used in bacterial Gram staining are ( ). A: Crystal violet B: Iodine solution C: Ethanol D: Safranine
- The following substances are used in Gram staining except A: iodine B: crystal violet C: alcohol D: safranin E: congo red
- Gram staining involves the following steps like: ( ) A: Make a slide of bacteria sample to be stained. B: Add the secondary stain safranin to the slide and incubate for 1 minute. C: Rinse sample or slide with acetone or alcohol for 30 seconds and rinse with a gentle stream of water. D: Add the primary stain (crystal violet) to the sample or slide and incubate for 1 minute.
- Which<br/>of the following buffer solutions have the largest buffer capacity? ( ) A: 1L 0.1 M HAc solution + 1L 0.2 M NaAc solution B: 1L 0.15 M HAc solution + 1L 0.15 M NaAc solution C: 1L 0.1 M HAc solution + 1L 0.1 M NaAc solution D: 1L 0.2 M HAc solution +1L 0.1 M NaAc solution