• 2021-04-14
    请阅读以下文献,思考后回答下列问题:MoreiraLA,etal.AWolbachiaSymbiontinAedesaegyptiLimitsInfectionwithDengue,Chikungunya,andPlasmodiumCell2009;139(7):1268–781)在1269页左半页最下面一段,Thesemosquitostrainswerecomparedtotetracycline-treatedcounterpartsthatweregeneticallyidenticalbutlackedtheWolbachiainfection,namedPGYP1.tetandPGYP1.out.tet,respectively.用四环素处理得到的蚊作为Wolbachia阴性样本有何潜在问题?实验设计上可以做何改进?2)在1269页右半页第二段,ToprovideamoreconservativetestofWolbachia-mediatedinterference,mosquitoeswereintrathoracicallyinjectedwithDENV-2.Theseexperimentscircumventedthemidgutbarriertoinfection(Woodringetal.,1996)andallowedforthedeliveryofarepeatableinoculatingdose(around2,750infectiousparticles/mosquito)ofDENV-2thatproducedconsistenthigh-titerinfectionsincontrolmosquitoes.这种胸内直接注射的方法有何优点?有何潜在的问题?是否人为地将可能影响Dengue感染的因素排除在考虑之外?这些被排除考虑的因素可能有哪些?3)检测全身的总病毒量后,为何还要分部位检测病毒量?哪个检测指标跟疾病的传播更有相关性?4)文章中检测了免疫相关的基因表达,并提出Wolbachia可能通过激活免疫应答而影响Dengue,Chikungunya以及Plasmodium的传播。你认为这一假说是否合理?Wolbachia作为稳定存在于蚊体内的共生菌,是否可能被免疫系统识别?作为对比,转基因小鼠通常会对转基因表达的抗原产生免疫应答吗?