When you doing transgenic plant, you could do overexpression, gene knock-down or gene knock out. Overexpression usually refers to an experiment when DNA is added to the cell to force expression of the gene to a much higher than normal level.()
举一反三
- which of following do not belong to pathogenic variations of the endogenous genes ( ) A: gene amplification B: abnormal gene expression C: invasion of the foreign genes D: gene arrangement E: point mutation
- Which of the following is correct about pro-oncogene? A: It is a normal cellular gene B: They are normally involved in the control of the cell cycle C: When mutated or inappropriately expressed,can cause a cell to become malignant D: All of the above
- The operator is A: Another name for the terminator of a gene B: A DNA site bound by repressor proteins C: A protein that coordinates gene expression D: A part in the leader region of mRNA
- Consider the TCR genes. The α gene has _______ V gene regions than does the γ, and the α <br/>gene has ______ C gene regions than does the β gene. A: fewer; fewer B: fewer; the same number C: more; fewer D: more; the same number
- A gene knock-in (or knock-in) refers to a genetic engineering method that involves the one-for-one substitution of DNA sequence information in a genetic locus or the insertion of sequence information not found within the locus. A gene knock-in therefore can be seen as a gain of function mutation and a gene knockout a loss of function mutation, but a gene knock-in may also involve the substitution of a functional gene locus for a mutant phenotype that results in some loss of function.