举一反三
- The DNA polymerases require a primer. A primer is a strand segment (complementary to the template) with a free 3′-hydroxyl group to which a nucleotide can be added; the free 3′ end of the primer is called the ______________.
- The 5'→3' exonuclease activity of intact DNA polymerase I can replace a segment of DNA (or RNA) paired to the template strand, in a process known as ____________________.
- Which of the following descriptions about DNA and RNA polymerase are correct: A: RNA polymerase synthesize RNA chain using nucleoside diphosphate instead of nucleoside triphosphates. B: RNA polymerase can initiate transcription without a primer, and nucleotides are added to 5’ end of the growing RNA chain. C: RNA polymerase can add nucleotides in both 3’ or 5’ end of RNA chain. D: Both RNA polymerase and DNA polymerase can only add nucleotides to 3’ end of the polynucleotides.
- What is the function of RNA primer during DNA replication? A: to provide a 3’ OH group for DNA polymerase B: to provide a 5’ OH group for DNA polymerase C: to provide a 3’ phosphate group for DNA polymerase D: to provide a 5’ phosphate group for DNA polymerase E: to provide a hydrogen bond for double strand of DNA .
- Simple<br/>set of rules for primer sequence design include( ) A: PCR<br/>primers should be 15-30 nucleotides in length. B: optimal GC content of the primer is 40%-60%. C: prefer one or two G or C at the 3’-end of primer. D: avoid primer self complementarities.
内容
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The role of RNA primer during DNA replication is A: to guide DNA polymerase to bind with DNA template B: to provide 5’ phosphate end C: to provide attachment sites for four kinds of NTP D: to provide 3’-OH end to initiate synthesis of new DNA
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Which<br/>of the following statements about DNA polymerase III is NOT true:() A: Deoxyribonucleotide<br/>are connected to 5’ hydroxyl of synthesized DNA by DNA pol III B: To<br/>catalyze deoxyribonucleotide connected to the primer C: Requires<br/>four kinds of 5’-dNTP D: To<br/>catalyze deoxyribonucleotide connected to 3’ terminal of RNA<br/>primer E: Pyrophosphate<br/>is one of the product
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Which of the following statements about DNA polymerase III is NOT true: A: Deoxyribonucleotide are connected to 5’ hydroxyl of synthesized DNA by DNA pol III B: Requires four kinds of 5’-dNTP C: To catalyze deoxyribonucleotide connected to 3’ terminal of RNA primer D: Pyrophosphate is one of the product
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The function of DNA polymerase I is to replace RNA primer with DNA. On which strand would you expect DNA polymerase I to be more active? A: the leading strand B: the lagging strand C: equally active on both strands D: neither , only DNA polymerase III is involved in synthesis
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The<br/>role of RNA primer during DNA replication is:() A: to<br/>guide DNA polymerase to bind with DNA template B: to<br/>provide 5’ phosphate end C: to<br/>provide attachment sites for four kinds of NTP D: inducing<br/>synthesis of RNA E: to<br/>provide 3’-OH end to initiate synthesis of new DNA