A primer is a strand segment (complementary to the template) with a free 3'-hydroxyl group to which a nucleotide can be added; the free 3' end of the primer is called the ______________________.
举一反三
- The DNA polymerases require a primer. A primer is a strand segment (complementary to the template) with a free 3′-hydroxyl group to which a nucleotide can be added; the free 3′ end of the primer is called the ______________.
- The 5'→3' exonuclease activity of intact DNA polymerase I can replace a segment of DNA (or RNA) paired to the template strand, in a process known as ____________________.
- Which of the following descriptions about DNA and RNA polymerase are correct: A: RNA polymerase synthesize RNA chain using nucleoside diphosphate instead of nucleoside triphosphates. B: RNA polymerase can initiate transcription without a primer, and nucleotides are added to 5’ end of the growing RNA chain. C: RNA polymerase can add nucleotides in both 3’ or 5’ end of RNA chain. D: Both RNA polymerase and DNA polymerase can only add nucleotides to 3’ end of the polynucleotides.
- What is the function of RNA primer during DNA replication? A: to provide a 3’ OH group for DNA polymerase B: to provide a 5’ OH group for DNA polymerase C: to provide a 3’ phosphate group for DNA polymerase D: to provide a 5’ phosphate group for DNA polymerase E: to provide a hydrogen bond for double strand of DNA .
- Simple<br/>set of rules for primer sequence design include( ) A: PCR<br/>primers should be 15-30 nucleotides in length. B: optimal GC content of the primer is 40%-60%. C: prefer one or two G or C at the 3’-end of primer. D: avoid primer self complementarities.