The DNA polymerases require a primer. A primer is a strand segment (complementary to the template) with a free 3′-hydroxyl group to which a nucleotide can be added; the free 3′ end of the primer is called the ______________.
举一反三
- A primer is a strand segment (complementary to the template) with a free 3'-hydroxyl group to which a nucleotide can be added; the free 3' end of the primer is called the ______________________.
- Simple<br/>set of rules for primer sequence design include( ) A: PCR<br/>primers should be 15-30 nucleotides in length. B: optimal GC content of the primer is 40%-60%. C: prefer one or two G or C at the 3’-end of primer. D: avoid primer self complementarities.
- Which is correct about DNA polymerase and RNA polymerase: A: RNA polymerases use nucleoside diphosphate instead of nucleoside triphosphate for synthesis of polynucleotide chains B: RNA polymerases need primer and add nucleotides in the 5 'end of the polynucleotide chain C: DNA polymerase can add nucleotides in both ends of the polynucleotide chain D: All of the RNA polymerases and DNA polymerases can only add nucleotides in the 3’ end of the polynucleotide chain
- What is the function of RNA primer during DNA replication? A: to provide a 3’ OH group for DNA polymerase B: to provide a 5’ OH group for DNA polymerase C: to provide a 3’ phosphate group for DNA polymerase D: to provide a 5’ phosphate group for DNA polymerase E: to provide a hydrogen bond for double strand of DNA .
- Which<br/>is correct about DNA polymerase and RNA polymerase:() A: RNA<br/>polymerases use nucleoside diphosphate instead of nucleoside<br/>triphosphate for synthesis of polynucleotide chains B: RNA<br/>polymerases need primer and add nucleotides in the 5 'end of the<br/>polynucleotide chain C: DNA<br/>polymerase can add nucleotides in both ends of the polynucleotide<br/>chain D: All<br/>of the RNA polymerases and DNA polymerases can only add nucleotides<br/>in the 3’ end of the polynucleotide chain