Simple set of rules for primer sequence design include( ) A: PCR primers should be 15-30 nucleotides in length. B: optimal GC content of the primer is 40%-60%. C: prefer one or two G or C at the 3’-end of primer. D: avoid primer self complementarities.

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2022-06-09 问题

Simple set of rules for primer sequence design include( ) A: PCR primers should be 15-30 nucleotides in length. B: optimal GC content of the primer is 40%-60%. C: prefer one or two G or C at the 3’-end of primer. D: avoid primer self complementarities.

Simple<br/>set of rules for primer sequence design include( ) A: PCR<br/>primers should be 15-30 nucleotides in length. B: optimal GC content of the primer is 40%-60%. C: prefer one or two G or C at the 3’-end of primer. D: avoid primer self complementarities.