Restriction endonuclease
Restriction endonuclease
6．限制性核酸内切酶（Restriction endonuclease）
6．限制性核酸内切酶（Restriction endonuclease）
The enzyme used in the polymerase chain reaction is ( ) A: restriction endonuclease B: reverse transcriptase C: DNA polymerase D: RNA polymerase
The enzyme used in the polymerase chain reaction is ( ) A: restriction endonuclease B: reverse transcriptase C: DNA polymerase D: RNA polymerase
Which of the following are NOT true about DNA polymerase I in E. coli: A: It can repair the gap in the single-strand DNA. B: It can replace DNA polymerase III to add dNTP into DNA chain when DNA template is damaged. C: Using dNTP as substrate. D: It possesses activity of 5’→3’ endonuclease.
Which of the following are NOT true about DNA polymerase I in E. coli: A: It can repair the gap in the single-strand DNA. B: It can replace DNA polymerase III to add dNTP into DNA chain when DNA template is damaged. C: Using dNTP as substrate. D: It possesses activity of 5’→3’ endonuclease.
Which step involves transformation? A: target DNA removed from cells and isolated B: cloning host takes up a plasmid C: target DNA and plasmid treated with the same restriction endonuclease D: desired protein is produced by cloning host E: gene is amplified by multiplication of cloning host
Which step involves transformation? A: target DNA removed from cells and isolated B: cloning host takes up a plasmid C: target DNA and plasmid treated with the same restriction endonuclease D: desired protein is produced by cloning host E: gene is amplified by multiplication of cloning host
Which<br/>of the following are NOT true about DNA polymerase I in E.coli:() A: It<br/>can repair the gap in the single-strand DN B: It<br/>can replace DNA polymerase III to add dNTP into DNA chain when DNA<br/>template is damaged. C: It<br/>possesses activity of 3’→5’ exonuclease. D: Using<br/>dNTP as substrate. E: It<br/>possesses activity of 5’→3’ endonuclease.
Which<br/>of the following are NOT true about DNA polymerase I in E.coli:() A: It<br/>can repair the gap in the single-strand DN B: It<br/>can replace DNA polymerase III to add dNTP into DNA chain when DNA<br/>template is damaged. C: It<br/>possesses activity of 3’→5’ exonuclease. D: Using<br/>dNTP as substrate. E: It<br/>possesses activity of 5’→3’ endonuclease.
在真核细胞DNA复制中( ) A: 只有一个复制起始点,只形成一个复制体 B: 冈崎片段长度为1000~2000个核苷酸 C: 复制泡一旦形成,解旋酶便从DNA上解离 D: 复制过程贯穿整个细胞周期 E: FEN1(flap endonuclease 1)和引物去除有关
在真核细胞DNA复制中( ) A: 只有一个复制起始点,只形成一个复制体 B: 冈崎片段长度为1000~2000个核苷酸 C: 复制泡一旦形成,解旋酶便从DNA上解离 D: 复制过程贯穿整个细胞周期 E: FEN1(flap endonuclease 1)和引物去除有关